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About Greyledge Technologies

Greyledge Technologies was established by Dr. David C. Karli to meet emerging physician and patient demands to facilitate and utilize mechanisms of natural healing to augment and/or support treatment of disease.  Dr. Karli also recognized the deficiency in product quality in the growing field and that Physicians didn’t know exactly what they were putting into their patients. In order to meet these first initiative, Greyledge has developed novel, manual laboratory-based methods to process autologous whole blood and bone marrow tissues to produce biologic preparations that are quality and quantity controlled to create a better product and higher level of research and development to advance the field of cellular therapies. 

Each of our biologic preparations is carefully analyzed prior to the point at which a physician returns it to the patient. This way, we ensure quality, safety and effectiveness and to meet physician-specified compositions. Greyledge’s approach is flexible, allowing physicians to optimize their practice of medicine through maintaining the highest standards in preparing, analyzing, applying and learning from autologous PRP and BMC preparations.

If you’re a patient looking for the optimal way to meet your needs, or if you’re a physician looking to integrate Regenerative Medicine seamlessly into your practice, now is the time to reach out to Greyledge. If you’re ready, we’re ready.

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Greyledge Technologies About Us

The Greyledge Mission 

Our mission is to leverage scientific advancements in creating and innovating quality controlled autologous biologic preparations, which enable physicians to optimize the practice of Regenerative Medicine.

Greyledge Technologies has created a technology platform that allows physicians the opportunity to integrate autologous PRP and BMC biologic preparations into their practice using a meticulously controlled cell processing facility, coupled with fully documented and validated sample analysis with every product produced.

The Greyledge Team

David Karli Headshot

David C. Karli, MD MBA

Founder and Chief Executive Officer

Greyledge operations are currently being led by company Founder and CEO – David Karli. He is responsible for all aspects of company development, including the integration of a strong team of professionals to support Greyledge’s innovative approach to regenerative medicine.

After attending medical school at the University of Maryland, Dr. Karli completed his residency in Physical Medicine and Rehabilitation at Harvard Medical School. Subsequently, he joined the faculty at Harvard, serving as an Attending Physician with the Department of PM&R at Spaulding Rehabilitation Hospital, as well as working in the Department of Orthopedic Surgery at Mass General Hospital.

After joining the Steadman Clinic in 2003, Dr. Karli began to adopt the use of autologous-based therapies that were emerging in the field of regenerative medicine. He has continued to lead the effort required to refine the practice of regenerative medicine, and has published pivotal clinical results and lectured extensively. His practice has grown substantially and includes treating elite-level and professional athletes. As a result, Dr. Karli has been featured in high-level media publications such as the Wall Street Journal, New York Times, ESPN Magazine and Denver Magazine.

Dr. Karli recognized early on that understanding the composition of regenerative biologic preparations used in treating his patients would be critical to refining his practice of medicine. He also realized that a “one size fits all” approach to creating the therapeutic preparations wouldn’t meet the need for an evidence-based, precision medicine approach. As a result, he founded Greyledge. In support of his concept for regenerative medicine, he completed the COLA Laboratory Director training course, and obtained his MBA (Daniels College of Business, University of Denver).

Greyledge has achieved sustained profitability and is ready for scalable expansion. Dr. Karli is positioning the company to maintain its unique technology platform offering by tracking regulatory activity, advancing biologic sample analytics and quality measures, and creating protocols that allow for customization of patient biologic preparations.

Zoe Bedford Karlli Headshot

Zoe Bedford Karli


Zoe Bedford Karli, our President, and a Director, officially joined the Greyledge executive team in 2019. She graduated from the University of Virginia via a Navy ROTC scholarship in 2005, served active military duty, and then transitioned into the world of business, working with both seasoned and start-up companies. She is a seasoned medical, biotechnical, and direct sales professional, with expertise in sales, e-commerce, distributorship, and operations. She has held territory leadership roles in pharmaceutical sales with Novartis, 2006-2009, medical device sales with Depuy Spine, and in conjunction co-developed a successful private distributorship managing independent contractors for multiple product lines within medical device, pharmaceuticals, cosmetic and dental specialties, 2009-2017. She then began working behind the scenes with Greyledge Technologies to officially assume a Directorship role in 2019.

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Jim Johnston

Chief of Corporate Development

Jim Johnston serves as Chief of Business Development for Greyledge, reporting to the CEO with responsibility for the company’s overarching strategy, including long-term planning, global business and corporate development, and strategic investments, acquisitions and divestitures.  Mr. Johnston has spent the past 21 years advising and investing in companies across a variety of industries and sizes.  He has significant experience in financing, mergers and acquisitions, strategic execution and board level decision making.  Jim was an investment banker at Goldman Sachs from 2000-2008 in Mergers and Acquisitions and Financial Sponsor Coverage.  He left as a Vice President and joined Nexphase Capital Partners (FKA Moelis Capital Partners) in 2008 where he ultimately ran the Industrials Group as a Partner.  In 2018, Mr. Johnston left Nexphase and founded Emerald Lake Advisors in order to focus his energy on investing and advising small entrepreneurial companies.

Mr. Johnston serves on the board of directors of the Red Fox Community School and as a board observer of Jame Rollform Products.  He received a BS in Commerce with Distinction from the University of Virginia in 1991 and was commissioned as a Naval Officer through the ROTC program.  After graduating Nuclear Power School and Prototype he served as a submarine officer and is a qualified Naval Nuclear Engineer.  After his separation from the Navy, he received his MBA from Harvard Business School in 2000.

Mr. Johnston is married with three sons and volunteers as a basketball and baseball coach in his local community.

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Richard C. Bedford

Director of Strategy and Innovation

Dick Bedford is a strategic thinker known for his ability to evaluate, deconstruct and solve complex problems.  He worked for NATO helping senior leaders develop strategy and generate solutions. A former naval officer, he spent 25 years in the U.S. Navy commanding both at sea and ashore, retiring as a Captain.  He served in a multitude of command and operational billets both in the US and Europe, and while flying, logged over 3800 hours in the F-14, to include 75 combat hours.

Mr. Bedford graduated from Tulane University in 1978 receiving a Bachelor of Science in Engineering; graduated from Harvard University in 1991, receiving a Master in Public Administration degree from the John F. Kennedy School of Government; and, from Georgetown University in 1998 where as a Moreau fellow he received a Master in National Security Studies from the Walsh School of Foreign Service.  He is also a seminar graduate of the National Defense University School for Information and Resources Management in Washington DC and a 2000 graduate of the Harvard University School for Senior Executive Fellows in Cambridge, MA.

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Mark D. Kitchen

VP of Operations

Mark D. Kitchen recently joined the Greyledge Technologies team as the Director of Operations.  Mark will help establish and lead many of our logistics, quality, and supporting processes as Greyledge Technologies expands its’ services throughout the United States.  Mark is a former US Army Lieutenant Colonel where he served in a wide range of assignments including Battalion Commander.  Upon returning to civilian life, he was instrumental in domestic and international leadership roles for semi-conductor tool manufacturing firm Celerity, Inc. (Austin, TX), including Plant Manager of the company’s start-up facility in Kuala Lumpur, Malaysia.  Mark was the Director of Operations and Material Management with Celling Biosciences (Austin, TX) for eight years before establishing consulting firm Kitchen & Associates (Houston, TX).

Mark received his Master’s Degree from The US Army Command and General Staff College at Fort Leavenworth, KS.  He also has a B.A. in Business Administration from Texas A&M University at Texarkana, TX.

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Phil Mansour


Mr. Mansour has been actively involved with disruptive technology as an inventor, manager, executive, and coach for more than 3 decades. He has extensive experience within many sectors – Bio-Technology, Consumer Electronics, Computer Science, Energy, Health Sciences, Hospitality, and Publishing. He has spoken often throughout his career on the future of technology and the effects of its disruption.

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Howard Ash


Howard Ash is an accomplished Executive with extensive experience in business and finance, who served as CEO, COO, and CFO to a variety of high-profile, international companies. Mr. Ash continues to serve as Chairman of Claridge Management since 2000. He serves as Chairman of the audit committee, a member of the board of directors, and a member of the Nominating and Governance Committee of  Ault Global (NYSE:DPW) 
Mr. Ash was a director of Net Element, Inc., (NASDAQ-NETE) from June 13, 2016, through July 13, 2020, serving as Chairman of both the Audit and Compensation committees, as well as the Nominating and Governance Committees during his tenure. He served as Chief Operating Officer of BioCard Corporation from 1997 to 2007. He served as Chief Operating Officer of CITA Americas, Inc. from 1996 to 1997. Mr. Ash served as Chief Executive Officer of the high profile IEDC Marketing, Inc. from 1992 to 1996. Mr. Ash currently serves on the Advisory Board of the UK-based E2Exchange, the Institute of Entrepreneurs, since 2011, and is the only non-UK citizen holding that position. Mr. Ash served from 2009 to 2014 in senior development and strategic capacity for One Laptop Per Child, a global NGO created to provide educational opportunities providing laptops to the world’s poorest children. Prior Chairmanships include 2009 through 2012 term for the Sturge Weber Foundation, a non-profit organization dedicated to curing this rare but fatal syndrome affecting children. Previously, Mr. Ash was an Advisory Board Member to Edge Global Investment Limited which forged a strategic partnership with the Africa Forum, consisting of 37 former Heads of State and Government. Mr. Ash started an interest-free micro-loan society in 1987 that has provided more than $15 million in micro-loans throughout the United States and Israel. In 1999, Mr. Ash founded the Circle of Life Resource Center, Inc., a food bank in Miami, Florida that feeds several hundred families per week. Howard earned a Bachelor of Commerce degree, with Honors in Accounting and Law from the University of Witwatersrand (South Africa) in 1980.
He continues to strive to leverage relationships on a personal and business level to create meaningful added value synergies.