Patient Data Research

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There are numerous reports on potential applications for PRP and BMCC in the basic science and clinical research literature, and that body of evidence continues to grow. Unfortunately, a significant majority of both clinical utilization and research of PRP and BMC used products which had very little quality control and very few cell counts.

At Greyledge, we believe that not knowing exactly what is being put into a patient during a cell therapy is unacceptable. Knowing these cell counts precisely and consistently in our products has created a tremendous opportunity for Greyldge to research the relationship between all of the cells and variables in these treatments to patient outcomes in a continual learning model. Only through careful measurement, data collection and study of dose response relationships can we learn to further optimize and customize our product for both the patient being treated and the problem we are targeting.

Greyledge has created a novel web-based data collection and management system that allows our clinical partners to capture this critical data before, during and after treatment. Data from our products and patient reported results of treatment can then be analyzed through application of AI (artificial intelligence) and ML (machine learning) algorithms that will speed up and enhance our ability to learn from every product made and every patient treated to advance the field of cellular therapies and apply what we learn to continually make our products and the delivery of them better in time.

Below is a list of possible illnesses and injuries where autologous preparations have been reported for treating a wide variety of pathologies with links to references provided.

JOINT INJECTION

Kon E, et al. (2010) Platelet-rich plasma: intra-articular knee injections produced favorable results on degenerative cartilage lesions.
http://www.ncbi.nlm.nih.gov/pubmed/19838676

Khoshbin  A, et al. (2013)  The efficacy of platelet-rich plasma in the treatment of symptomatic knee osteoarthritis: a systematic review with quantitative synthesis. http://www.ncbi.nlm.nih.gov/pubmed/24286802

Sánchez M, et al. (2012) Ultrasound-guided platelet-rich plasma injections for the treatment of osteoarthritis of the hip.
http://rheumatology.oxfordjournals.org/content/51/1/144.long

Centeno C, et al. (2016)  A dose response analysis of a specific bone marrow concentrate treatment protocol for knee osteoarthritis.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575428/

IN CONJUNCTION WITH SURGICAL TREATMENT

Fortier LA, et al. (2010) Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in an equine model.
http://www.ncbi.nlm.nih.gov/pubmed/20720135

Milano G, et al. (2010) The effect of platelet rich plasma combined with microfractures on the treatment of chondral defects: an experimental study in a sheep model. http://www.ncbi.nlm.nih.gov/pubmed/20433936

Buda R, et al. (2015) One-step bone marrow-derived cell transplantation in talar osteochondral lesions: mid-term results.
http://www.ncbi.nlm.nih.gov/pubmed/25606518

Foster TE, et al. (2009) Platelet-rich plasma: from basic science to clinical application.
http://www.ncbi.nlm.nih.gov/pubmed/19875361

Murray MM, et al. (2009) Enhanced histologic repair in a central wound in the anterior cruciate ligament with a collagen–platelet-rich plasma scaffold.
http://www.ncbi.nlm.nih.gov/pubmed/17415785

Braun HJ, et al. (2013) The Use of PRP in ligament and meniscal healing.
http://www.ncbi.nlm.nih.gov/pubmed/24212368

Yu H, et al. (2015) Meniscus repair using mesenchymal stem cells – a comprehensive review.
http://www.ncbi.nlm.nih.gov/pubmed/25925426

Cook JL, et al. (2016) Multiple injections of leukoreduced platelet rich plasma reduce pain and functional impairment in a canine model of ACL and meniscal deficiency. http://www.ncbi.nlm.nih.gov/pubmed/26403590

LATERAL EPICONDYLITIS

Peerbooms JC, (2010) Positive effect of an autologous platelet concentrate in lateral epicondylitis in a double-blind randomized controlled trial: platelet-rich plasma versus corticosteroid injection with a 1-Year follow-up. http://www.ncbi.nlm.nih.gov/pubmed/20448192

Gosens T, et al. (2011) Ongoing positive effect of platelet-rich plasma versus corticosteroid injection in lateral epicondylitis: a double-blind randomized controlled trial with 2-year follow-up. http://www.ncbi.nlm.nih.gov/pubmed/21422467

Mishra A, et al. (2014) Efficacy of platelet-rich plasma for chronic tennis elbow: a double-blind, prospective, multicenter, randomized controlled trial of 230 patients. http://www.ncbi.nlm.nih.gov/pubmed/23825183

PATELLAR TENDONITIS

Cervellin M, et al. (2012) Autologous platelet-rich plasma gel to reduce donor-site morbidity after patellar tendon graft harvesting for anterior cruciate ligament reconstruction: a randomized, controlled clinical study. http://www.ncbi.nlm.nih.gov/pubmed/21678095

Di Matteo B, et al. (2015) Platelet-rich plasma: evidence for the treatment of patellar and Achilles tendinopathy—a systematic review.
http://www.ncbi.nlm.nih.gov/pubmed/25323041

Pascual-Garrido C, et al. (2012) Treatment of chronic patellar tendinopathy with autologous bone marrow stem cells: a 5-year followup.
http://www.ncbi.nlm.nih.gov/pubmed/22220180

ROTATOR CUFF TEARS

Randelli P, et al. (2011) Platelet rich plasma in arthroscopic rotator cuff repair: a prospective RCT study, 2-year follow-up.
http://www.ncbi.nlm.nih.gov/pubmed/21570659

Hernigou P, et al. (2014) Biologic augmentation of rotator cuff repair with mesenchymal stem cells during arthroscopy improves healing and prevents further tears: a case-controlled study. http://www.ncbi.nlm.nih.gov/pubmed/24913770

ACHILLES TENDINOPATHY

Sanchez M, et al. (2007) Comparison of surgically repaired Achilles tendon tears using platelet rich fibrin matrices.
http://www.ncbi.nlm.nih.gov/pubmed/17099241

Stein BE, et al. (2015) Outcomes of acute Achilles tendon rupture repair with bone marrow aspirate concentrate augmentation.
http://www.ncbi.nlm.nih.gov/pubmed/25795246

Galasso O, et al. (2008) Expandable intramedullary nailing and platelet rich plasma to treat long bone non-unions.
http://www.ncbi.nlm.nih.gov/pubmed/19384608

Sampson S, et al. (2008) Platelet rich plasma injection grafts for musculoskeletal injuries: a review.
http://www.ncbi.nlm.nih.gov/pubmed/19468902

Pettine K, et al. (2015) Percutaneous Injection of Autologous Bone Marrow Concentrate Cells Significantly Reduces Lumbar Discogenic Pain Through 12 Months. http://onlinelibrary.wiley.com/doi/10.1002/stem.1845/abstract;jsessionid=4B093BB048A7B7E6ED042103925DBB2A.f03t02

Pettine K, et al. (2016) Treatment of discogenic back pain with autologous bone marrow concentrate injection with minimum two year follow-up.
https://www.ncbi.nlm.nih.gov/pubmed/26156727

Monfett M, et al. (2016) Intradiscal platelet-rich plasma (PRP) injections for discogenic low back pain: an update.
https://www.ncbi.nlm.nih.gov/pubmed/27073034

Murphy M, et al. (2013) Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine.
Cassano JM, et al. (2016) Bone marrow concentrate and platelet-rich plasma differ in cell distribution and interleukin 1 receptor antagonist protein concentration. http://www.ncbi.nlm.nih.gov/pubmed/26831858
Wang SW, et al. (2014) Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis. http://www.ncbi.nlm.nih.gov/pubmed/24578232
Cicha I, et al. (2004) Activated human platelets release connective tissue growth factor.
De Mos M, et al. (2008) Can platelet-rich plasma enhance tendon repair?
Evans, C. (1999) Cytokines and the role they play in the healing of ligaments and tendons.
Kumar V, and JR Chapman. (2008) Autologous thrombin: intraoperative production from whole blood.
Marx RE, et al. (1998) Platelet-rich plasma: growth factor enhancement for bone grafts.
Weibrich G, et al. (2004) Effect of platelet concentration in platelet-rich plasma on the peri-implant bone regeneration.
Zechner W, et al. (2003) Influence of platelet-rich plasma on osseus healing of dental implants: a histologic and histomorphometric study in minipigs.
Woodell-May J, et al. (2011) Autologous protein solution inhibits MMP-13 production by IL-1β and TNFα-stimulated human articular chondrocytes.
van Buul GM, et al. (2011) Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes.

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